Plasma adiponectin levels and sonographic phenotypes of subclinical carotid artery atherosclerosis : Data from the SAPHIR study

Copyright: Copyright 2008 Elsevier B.V., All rights reserved.Background and Purpose - Adipose tissue produces and secretes a number of bioactive molecules, conceptualized as adipocytokines. Adiponectin has been identified as one of the adipocytokines, and hypoadiponectinemia was demonstrated in patients with obesity, diabetes mellitus, and coronary artery disease. Whether decreased adiponectin levels are cause or consequence is an important issue in the discussion on the association between adiponectin and atherosclerosis. In the present study, we investigated the association of plasma adiponectin levels with sonographic phenotypes of subclinical atherosclerosis, which may represent different stages of disease as well as common and distinct determinants. Methods - A total of 1515 middle-aged healthy white subjects (940 males and 575 females) were included. Common carotid artery intima-media thickness (CIMT) and presence of atherosclerotic plaques were assessed by B-mode ultrasound. Results - After adjustment for established risk factors, per 1 μg/mL decrease in adiponectin CIMT increased on the average by 3.48 μ in males (95% CI, 1.23 to 5.73 μm) and by 2.39 μ in females (95% CI, 0.50 to 4.27 μm). After dichotomizing adiponectin levels at the median and adjustment for established risk factors, the mean difference of CIMT between subjects with low and high adiponectin levels was 20.42 μm in men (95% CI, 6.80 to 34.04; P=0.003) and 20.75 μ in women (95% CI, 1.08 to 40.42; P=0.039). No significant relationship was found between adiponectin levels and presence of atherosclerotic plaques. Conclusion - Our results demonstrate an independent negative association of adiponectin levels and CIMT, whereas no relationship with presence of atherosclerotic plaques was found, thus suggesting hypoadiponectinemia as a risk factor in the development of early atherosclerosis.publishersversionPeer reviewe

Response to letter by Pilz et al

Copyright: Copyright 2008 Elsevier B.V., All rights reserved.publishersversionPeer reviewe

The Serifos Metamorphic Core Complex (Greece) — kinematic investigations of the southern detachment mylonites

The island of Serifos is situated about 100km SSE of Athens in the Aegean Sea and belongs to the Attic-Cycladic massif. The geology of Serifos is largely characterized by a shallow hornblendebiotite granodiorite pluton that intruded in the late Miocene into a previously deformed (under blueschist conditions) sequence mainly consisting of ortho- and paragneisses, calc-silicate marbles, amphibolites and schists. The pluton has a dome-shaped body occupying the central and southern parts of the island (Salemink 1985). The Serifos MCC is the very western continuation of a zone of syn- to post tectonic intrusions younging from the East (Naxos, Paros ?12Ma) to the West (Serifos 9– 8Ma). Whereas the older intrusions in the East show a top to the North geometry, the Serifos MCC has developed a South-directed low-angle detachment fault...conferenc

Late stage evolution of the Serifos Metamorphic Core Complex (Cyclades, Greece)

The island of Serifos is located in the Western Cyclades within the Attic- Cycladic metamorphic belt. It represents the westward continuation of an arcuate belt of Metamorphic Core Complexes with intrusions of late syn-post tectonic intrusions younging from East (e.g. Naxos main activity ca. 12Ma) to West (e.g. Serifos with 9–8Ma). In scientific discussions the dominance of probably continuous extension since ca. 30Ma (e.g. Jolivet & Faccenna, 2000) and the presence of Metamorphic Core Complexes (Lister et al. 1984) is accepted. The speculated roll-back of the subducting plate possibly started due to the slowing down of absolute plate convergence rate between Africa and Eurasia. This model is attractive, because it would also explain the shift from a compressional Andean-type regime to an extensional Mariana-type regime (Jolivet & Faccenna 2000). Contrary to the kinematic directions reported from the Central and Eastern Cyclades, the movement of the hanging wall of the Serifos Metamorphic Core Complex is south directed. The island’s main part is occupied by an undeformed granodiorite. Early granitic intrusions intruded into low-grade M2-crystalline rocks that have been overprinted to as high as amphibolite facies conditions due to contact metamorphism. Parts of these rocks (gneisses and amphibolites) as well as the early intrusions are deformed to mylonites (Grasemann et al. 2004).conferenc

Kinematics and deformation structures in a crustal-scale shear zone on Kea (W. Cyclades, Greece)

It is generally agreed upon that the exhumation of metamorphic rocks in the Aegean is caused by post orogenic extension in the late Oligocene to early Miocene. This extension is in principle largely accommodated by low-angle crustal detachment faulting possibly resulting in the formation of metamorphic core complexes (MCC). Here, we present data from recent structural investigations on the island of Kea in the W. Cyclades, Greece. Our work focussed in the north of the island. Of the ca. 270m total structural thickness that was mapped, the entire section of rocks are highly strained. Exhumation during progressive deformation is recorded by the transition from ductile to brittle/ductile to brittle conditions. The regional characteristics and types of deformation structures vary depending on the protolith and the intensity of strain...conferenc

Sequencing patterns of ventilatory indices in less trained adults

Submaximal ventilatory indices, i.e., point of optimal ventilatory efficiency (POE) and anaerobic threshold (AT), are valuable indicators to assess the metabolic and ventilatory response during cardiopulmonary exercise testing (CPET). The order in which the ventilatory indices occur (ventilatory indices sequencing pattern, VISP), may yield additional information for the interpretation of CPET results and for exercise intensity prescription. Therefore, we determined whether different VISP groups concerning POE and AT exist. Additionally, we analysed fat metabolism via the exercise intensity eliciting the highest fat oxidation rate (Fatmax) as a possible explanation for differences between VISP groups. 761 less trained adults (41–68 years) completed an incremental exercise test on a cycle ergometer until volitional exhaustion. The ventilatory indices were determined using automatic and visual detection methods, and Fatmax was determined using indirect calorimetry. Our study identified two VISP groups with a lower work rate at POE compared to AT in VISPPOE < AT but not in group VISPPOE = AT. Therefore, training prescription based on POE rather than AT would result in different exercise intensity recommendations in 66% of the study participants and consequently in unintended physiological adaptions. VISPPOE < AT participants were not different to VISPPOE = AT participants concerning VO2peak and Fatmax. However, participants exhibiting a difference in work rate (VISPPOE < AT) were characterized by a higher aerobic capacity at submaximal work rate compared to VISPPOE = AT. Thus, analysing VISP may help to gain new insights into the complex ventilatory and metabolic response to exercise. But a methodological framework still must be established

Extensional crustal-scale shear zones in the Western Cyclades (Kea, Greece)

Intense seismicity and intensely developed active and ancient fault systems are common to the Aegean Region. Extending/ thinning crust involves a complex interplay of (1) Gulf of Corinth riftexpansion, (2) west- and south-ward retreat of the Hellenic Trench, (3) westward impingement of the Anatolian Platen, and/or (4) propagation of the Anatolian Fault system into the Aegean. New geological/structural investigations on Kea (also known as Tzia), in the Western Cyclades reveal a low angle crustal-scale, detachment-type ductile shear zone probably formed during Miocene extension and thinning of the continental crust...conferenc

The Mitochondrial T16189C Polymorphism Is Associated with Coronary Artery Disease in Middle European Populations

BACKGROUND: The pivotal role of mitochondria in energy production and free radical generation suggests that the mitochondrial genome could have an important influence on the expression of multifactorial age related diseases. Substitution of T to C at nucleotide position 16189 in the hypervariable D-loop of the control region (CR) of mitochondrial DNA (mtDNA) has attracted research interest because of its suspected association with various multifactorial diseases. The aim of the present study was to compare the frequency of this polymorphism in the CR of mtDNA in patients with coronary artery disease (CAD, n = 482) and type 2 diabetes mellitus (T2DM, n = 505) from two study centers, with healthy individuals (n = 1481) of Middle European descent in Austria. METHODOLOGY AND PRINCIPAL FINDINGS: CR polymorphisms and the nine major European haplogroups were identified by DNA sequencing and primer extension analysis, respectively. Frequencies and Odds Ratios for the association between cases and controls were calculated. Compared to healthy controls, the prevalence of T16189C was significantly higher in patients with CAD (11.8% vs 21.6%), as well as in patients with T2DM (11.8% vs 19.4%). The association of CAD, but not the one of T2DM, with T16189C remained highly significant after correction for age, sex and body mass index (BMI) and was independent of the two study centers. CONCLUSIONS AND SIGNIFICANCE: Our results show for the first time a significant association of T16189C with CAD in a Middle European population. As reported in other studies, in patients with T2DM an association with T16189C in individuals of European decent remains questionable

Mitochondrial DNA haplogroup T is associated with coronary artery disease and diabetic retinopathy: a case control study

<p>Abstract</p> <p>Background</p> <p>There is strong and consistent evidence that oxidative stress is crucially involved in the development of atherosclerotic vascular disease. Overproduction of reactive oxygen species (ROS) in mitochondria is an unifying mechanism that underlies micro- and macrovascular atherosclerotic disease. Given the central role of mitochondria in energy and ROS production, mitochondrial DNA (mtDNA) is an obvious candidate for genetic susceptibility studies on atherosclerotic processes. We therefore examined the association between mtDNA haplogroups and coronary artery disease (CAD) as well as diabetic retinopathy.</p> <p>Methods</p> <p>This study of Middle European Caucasians included patients with angiographically documented CAD (n = 487), subjects with type 2 diabetes mellitus with (n = 149) or without (n = 78) diabetic retinopathy and control subjects without clinical manifestations of atherosclerotic disease (n = 1527). MtDNA haplotyping was performed using multiplex PCR and subsequent multiplex primer extension analysis for determination of the major European haplogroups. Haplogroup frequencies of patients were compared to those of control subjects without clinical manifestations of atherosclerotic disease.</p> <p>Results</p> <p>Haplogroup T was significantly more prevalent among patients with CAD than among control subjects (14.8% vs 8.3%; p = 0.002). In patients with type 2 diabetes, the presence of diabetic retinopathy was also significantly associated with a higher prevalence of haplogroup T (12.1% vs 5.1%; p = 0.046).</p> <p>Conclusion</p> <p>Our data indicate that the mtDNA haplogroup T is associated with CAD and diabetic retinopathy in Middle European Caucasian populations.</p

Predictive value for cardiovascular events of common carotid intima media thickness and its rate of change in individuals at high cardiovascular risk - Results from the PROG-IMT collaboration.

AIMS: Carotid intima media thickness (CIMT) predicts cardiovascular (CVD) events, but the predictive value of CIMT change is debated. We assessed the relation between CIMT change and events in individuals at high cardiovascular risk. METHODS AND RESULTS: From 31 cohorts with two CIMT scans (total n = 89070) on average 3.6 years apart and clinical follow-up, subcohorts were drawn: (A) individuals with at least 3 cardiovascular risk factors without previous CVD events, (B) individuals with carotid plaques without previous CVD events, and (C) individuals with previous CVD events. Cox regression models were fit to estimate the hazard ratio (HR) of the combined endpoint (myocardial infarction, stroke or vascular death) per standard deviation (SD) of CIMT change, adjusted for CVD risk factors. These HRs were pooled across studies. In groups A, B and C we observed 3483, 2845 and 1165 endpoint events, respectively. Average common CIMT was 0.79mm (SD 0.16mm), and annual common CIMT change was 0.01mm (SD 0.07mm), both in group A. The pooled HR per SD of annual common CIMT change (0.02 to 0.43mm) was 0.99 (95% confidence interval: 0.95-1.02) in group A, 0.98 (0.93-1.04) in group B, and 0.95 (0.89-1.04) in group C. The HR per SD of common CIMT (average of the first and the second CIMT scan, 0.09 to 0.75mm) was 1.15 (1.07-1.23) in group A, 1.13 (1.05-1.22) in group B, and 1.12 (1.05-1.20) in group C. CONCLUSIONS: We confirm that common CIMT is associated with future CVD events in individuals at high risk. CIMT change does not relate to future event risk in high-risk individuals